Treponema Lecithinolyticum Lipopolysaccharide에 의한 matrix metalloproteinase-9의 발현
Effect of Treponema Lecithinolyticum Lipopolysaccharide on matrix metalloproteinase-9 expression
소속 상세정보
남정아
문선영/이진욱/차정헌/최봉규/유윤정
KMID : 0363020050350030675
Abstract
Bone resorption involves sequential stages of osteoclast precursor migration and differentiation of osteoclast precursors into multinucleated osteoclasts. Stromal cell derived factor
(SDF)-1 is a chemotactic factor for osteoclast precursor migration. Matrix metalloproteinase
(MMP)-9 is involved in migration of osteoclast precursors and activation of interleukin(IL)-1β.
Alveolar bone destruction is a characteristic feature of periodontal disease. Treponema
lecithinolyticum is a oral spirochete isolated from the periodontal lesions. The effect of
lipopolysaccharide(LPS) from T. lecithinolyticum on expression of SDF-1 and MMP-9 was
examined in cocultures of bone marrow cells and osteblasts derived from mouse calvariae.
T. lecithinolyticum LPS increased expression of MMP-9 in the coculture. Polymyxin B, an
inhibitor of LPS, abolished the increase of MMP-9 mRNA expression by LPS. LPS did not
increase the expression of SDF-1, IL-1β and tumor necrosis factor(TNF)-α mRNA in
cocultures. Prostaglandin E2(PGE2) up-regulated the expression of MMP-9 and NS398, an
inhibitor of PGE2 synthesis, down-regulated the induction of MMP-9 expression by T.
lecithinolyticm LPS.
These results suggest that T. lecithinolytium LPS increases MMP-9 expression in bone cells
via PGE2 and that the induction of MMP-9 expression by T. lecithinolyticum LPS is involved
in alveolar bone destruction of periodontitis patients by the increase of osteoclast precursor
migration and the activation of bone resorption-inducing cytokine.2)
키워드
Treponema Lecithinolyticum;Lipopolysaccharide;MMP-9
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